|
週次 |
日期 |
單元主題 |
|
第1週 |
3/6 |
1. Course introduction
2. Protein structure (Chap 2): introduce amino acids, protein secondary structure
3. Build up secondary structures using amino acid models (Practice, homework)
|
|
第2週 |
3/13 |
1. (Bring computer) Use molecular modeling software to display your protein (SwissPDBviewer) (Practice, homework)
2. Topology and motif
3. Protein folding theory, protein structural stability, protein folding kinetics |
|
第3週 |
3/20 |
1. Chaperone
2. Protein misfolding, amyloid, misfolding-related diseases |
|
第4週 |
3/27 |
Understanding cell biology from structural biology aspect (Chap 11)
Importance of visualization
Seeing molecular mechanism in cell signaling
How protein kinases work
|
|
第5週 |
4/3 |
民族掃墓節調整放假 |
|
第6週 |
4/10 |
Protein expression
Basic concept of protein biogenesis
Practical consideration of recombinant protein production
What can you do if you doesn’t get your protein
|
|
第7週 |
4/17 |
Protein crystallography
The importance of resolution
How to visualize/evaluate the result
General concept of protein crystallography
What protein crystallography can and cannot do
|
|
第8週 |
4/24 |
期中考 |
|
第9週 |
5/01 |
Introduce membrane protein (Chap 10)
Preparation of membrane proteins for structural studies
|
|
第10週 |
5/08 |
Use NMR to solve protein structure
Sample requirement, sample preparation, limitation of the technique, isotope-labeling)
(Practice) How to assign a 2D-NMR spectrum
How to use NMR to examine protein-protein or protein-ligand interaction
Hydrogen-deuterium exchange NMR and mass spectrometry (calculate protection factor)
|
|
第11週 |
5/15 |
Use cross-linking technique and mass spectrometry to study protein-protein interaction |
|
第12週 |
5/22 |
因生物物理化學年會停開, 後面課程前移 |
|
第12週 |
5/22 new |
Introduce intrinsically disordered protein (IDP)
Protein structure prediction (secondary structures, IDP, amyloid)
|
|
第13週 |
5/29 |
(Bring computer) Use molecular modeling software to make a beautiful structure illustration for paper publication (Pymol) (Practice) |
|
第14週 |
6/5 |
Cryo EM
Theory and importance.
Sample preparation
Cryo-EM applications
Example case: SARS-CoV-2 RNA polymerase
|
|
第15週 |
6/12 |
課程總檢討 (來不及上完的課也可能用到這週)
及自由討論作業時間 |
|
第16週 |
6/19 |
期末考 |